Effects of lysophosphatidic acid on human colon cancer cells and its mechanisms of action

Figure 2 LPA effect on SW480 cell proliferation. Results presented as mean ± SE, n = 5. A: Dose and time effect of LPA on the proliferation of SW480 cells. SW480 cells were starved in serum-free DMEM for 12 h and treated with LPA at different doses. At different time points, MTT assay was performed to evaluate cell numbers. ^bP < 0.001 vs 0 μmol/L LPA; B: Inhibitors blocked LPA-induced cell proliferation. SW480 cells were starved in serum-free DMEM for 12 h and treated with LPA (10 μmol/L). Inhibitors including LY294002 (50 μmol/L), PD98059 (10 μmol/L), and Y-27632 (10 μmol/L) were applied to cells 30 min before the action of LPA. ninety-six hours later, MTT assay was performed to evaluate cell growth. ^bP < 0.001 vs 10 μmol/L LPA.