Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells

Figure 7 Effects of FeNTA on HCV core and NS5A mRNA and protein levels. A: Core mRNA levels in Con1 cells treated with FeNTA and with or without deferoxamine; B: Core protein levels in Con1 cells treated with FeNTA and with or without deferoxamine; C: NS5A mRNA levels in Con1 cells treated with FeNTA and with or without deferoxamine; D: NS5A protein levels in Con1 cells treated with FeNTA and with or without deferoxamine. Data are presented as mean ± SE from triplicate samples, all normalized to GAPDH in the same samples. ^aP < 0.05 vs control. The Con1 full length HCV replicon cells were treated with indicated concentrations of FeNTA and with or without deferoxamine. After 24 h, cells were harvested and total RNA and proteins were extracted. Levels of mRNA were measured by quantitative RT-PCR, and protein levels were determined by Western blots as described in Materials and Methods. Values for cells without any treatment were set equal to 1.