Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells

Figure 6 Effects of deferoxamine on intracellular ROS induced by FeNTA in Huh-7, 9-13, and CNS3 cells. A: Fluorescence intensity with the H₂DCF-DA probe in Huh-7 cells; B: Fluorescence intensity with the H₂DCF-DA probe in 9-13 cells; C: Fluorescence intensity with the H₂DCF-DA probe in CNS3 cells; D: Fluorescence intensity with the (control) DCF-DA in CNS3 cells. Cells were loaded with 100 μmol/L H₂DCF-DA for 30 min, treated with different concentrations of deferoxamine (50, 100, 200 μmol/L) for 30 min, and then exposed to different concentrations of FeNTA (50, 100 μmol/L) for 1 h. Intracellular ROS production was measured as described in Materials and Methods.