Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells

Figure 1 Iron up-regulates HMOX1 mRNA levels in Huh-7, 9-13 and CNS3 cells. A: HMOX1 mRNA levels in Huh-7, 9-13 and CNS3 cells; B: HMOX1 mRNA levels in Huh-7 cells treated with 100 μmol/L FeNTA or 1 mmol/L H₂O₂ for 6 h; C: HMOX1 mRNA levels in 9-13 cells treated with 100 μmol/L FeNTA or 1 mmol/L H₂O₂ for 6 h; D: HMOX1 mRNA levels in CNS3 cells treated with 100 μmol/L FeNTA or 1 mmol/L H₂O₂ for 6 h. Values for cells without any treatment were set equal to 1. HMOX1 mRNA data are presented as means ± SE from triplicate samples, all normalized to GAPDH in the same samples. ^aP < 0.05 vs control. Huh-7, 9-13 or CNS3 cells were cultured as described in Materials and Methods, and treated with 100 μmol/L FeNTA or 1 mmol/L H₂O₂ for 6 h. Cells were harvested and total RNA was extracted. Levels of mRNA for HMOX1 and GAPDH were measured by quantitative RT-PCR.