Peroxisome proliferator-activated receptor-γ is essential in the pathogenesis of gastric carcinoma

Figure 9 PPARγ after pSi transfection. A: PPARγ mRNA expression in various cells detected by RT-PCR 48 h after transfection; B: PPARγ protein expression in various cells 48 h after transfection as detected by Western blotting. Lane M: 100 bp DNA marker, 500 bp was the brightest; Lane 1: MGC803-N (normal MGC803 cells); Lane 2: MGC803-HK (MGC803 cells transfected by empty vector); Lane 3: MGC803-pSi3 (MGC803 cells transfected by pSi3 vector); Lane 4: MGC803-pSi2 (MGC803 cells transfected by pSi2 vector); Lane 5: MGC803-pSi1 (MGC803 cells transfected by pSi1 vector). The effect of pSi1 was best in silencing PPAR-γ mRNA and protein expression in MGC803 cells.