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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Aug 14, 2009; 15(30): 3799-3806
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3799
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3799
Table 1 Primers and size of PCR products
| Methods | Primers | Sequence | Length (bp) |
| QRT-PCR | PTCH1 | 5'-TGTGCGCTGTCTTCCTTCTG-3' | 119 |
| 5'-ACGGCACTGAGCTTGATTC-3' | |||
| β-actin | 5'-GCCATCCTGCGTCG-3' | 260 | |
| 5'-TGGGCACCGGAACCGCT-3' | |||
| BSP | 5'-GGGAGTATTTGGGTGGTATATT-3' | 351 | |
| 5'-AAAAAACTACAAAAAAACACCACCTTTC-3' | |||
| MSP | Methylation | 5'-GAGGGATCGATACGAATTC-3' | 143 |
| 5'-GAAAACGCGAAAAAACTAAA-3' | |||
| Non-methylation | 5'-GAGGGATTGATATGAATTT-3' | 143 | |
| 5'-AAAAACACAAAAAAACTAAA-3' |
- Citation: Du P, Ye HR, Gao J, Chen W, Wang ZC, Jiang HH, Xu J, Zhang JW, Zhang JC, Cui L. Methylation of PTCH1a gene in a subset of gastric cancers. World J Gastroenterol 2009; 15(30): 3799-3806
- URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3799.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.3799