Methylation of PTCH1a gene in a subset of gastric cancers

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Aug 14, 2009 (publication date) through Jul 31, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 14, 2009; 15(30): 3799-3806
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3799

Table 1 Primers and size of PCR products

Methods	Primers	Sequence	Length (bp)
QRT-PCR	PTCH1	5'-TGTGCGCTGTCTTCCTTCTG-3'	119
		5'-ACGGCACTGAGCTTGATTC-3'
	β-actin	5'-GCCATCCTGCGTCG-3'	260
		5'-TGGGCACCGGAACCGCT-3'
BSP		5'-GGGAGTATTTGGGTGGTATATT-3'	351
		5'-AAAAAACTACAAAAAAACACCACCTTTC-3'
MSP	Methylation	5'-GAGGGATCGATACGAATTC-3'	143
		5'-GAAAACGCGAAAAAACTAAA-3'
	Non-methylation	5'-GAGGGATTGATATGAATTT-3'	143
		5'-AAAAACACAAAAAAACTAAA-3'

QRT-PCR: Quantitative PCR.

Citation: Du P, Ye HR, Gao J, Chen W, Wang ZC, Jiang HH, Xu J, Zhang JW, Zhang JC, Cui L. Methylation of PTCH1a gene in a subset of gastric cancers. World J Gastroenterol 2009; 15(30): 3799-3806
URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3799.htm
DOI: https://dx.doi.org/10.3748/wjg.15.3799