IL-10 and TNF-α promoter haplotypes are associated with childhood Crohn’s disease location

Table 2 Characteristic of the primers used to genotype the SNP in TNF-α, IL-10 and GR genes

Locus	SNP	Amplimers		ASO probe1
TNF-α		F 5'CTAAGGAATGGAGGGAGGGA3'
		R 5'CTTCTGTCTCGGTTTCTTCT3'
	T-1031C		rs1799964	GAGAAGATGAAGGAA
				GAGAAGACGAAGGAA
	C-863A		rs1800630	GACCCCCCCTTAACG
				GACCCCCACTTAACG
	C-857T		rs1799724	CCCTTAATGAAGACA
				CCCTTAACGAAGACA
	G-308A		rs59729336	GGGCATGGGGACGGG
				GGGCATGAGGACGGG
	G-238A		rs361525	CGGAATCGGAGCAGG
				CGGAATCAGAGCAGG
IL-10		F 5'CACTACTAAGGCTTCTTTGGG3'
		R 5'CTGTAGGAAGCCAGTCTCTG3'
	G-1087A		rs1800896	TTTGGGAGGGGGAAG
				TTTGGGAAGGGGAAG
	C-824T		rs1800871	GTGATGTAACATCTCTGTG
				GTGATGTAATATCTCTGTG
	C-597A		rs1800872	CGCCTGTCCTGTAGG
				CGCCTGTACTGTAGG
GR	R23K	F 5'TGTAGGATTGATATTCACTGATG3'	rs6189	GGAGAGGGGAGATGT
	G > A	R 5'CAAAAGTCTTCGCTGCTTGG3'		GGAGAAGGGAGATGT
	N363S	F 5'TCCATGGTGTGAGTACCTCTGG3'	rs6195	CGGTTCCGAAAGTTG
	A > G	R 5'GACCAGGGAAGTTCAGAGTCC3'		CGGTTCCGAAAATTG

ASO: Allele specific oligonucleotide.

¹Boldface characters indicate the polymorphic position.