Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

doi:10.3748/wjg.15.3757

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 14, 2009; 15(30): 3757-3766
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3757

Table 1 Interfering sequence specified for STAT3 gene

Number	Sequence
LV-STAT3siRNA-1	Oligo1: 5’TaaTGTTCTCTATCAGCACAATTTCAAGAGAATTGTGCTGATAGAGAACATTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaTGTTCTCTATCAGCACAATTCTCTTGAAATTGTGCTGATAGAGAACATTA3’
LV-STAT3siRNA-2	Oligo1: 5’TaaCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCATCTGCCTAGATCGGCTATCTCTTGAATAGCCGATCTAGGCAGATGTTA3’
LV-STAT3siRNA-3	Oligo1: 5’TaaCTTCAGACCCGTCAACAAATTCAAGAGATTTGTTGACGGGTCTGAAGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCTTCAGACCCGTCAACAAATCTCTTGAATTTGTTGACGGGTCTGAAGTTA 3’

Three coding regions corresponding to targeting human STAT3 starting at 466, 1638, and 1061 in the GenBank (NM 139276). Interfering sequence specified for STAT3 gene, namely LV-STAT3siRNA-1, LV-STAT3siRNA-2 and LV- STAT3siRNA-3, respectively.

Citation: Yang G, Huang C, Cao J, Huang KJ, Jiang T, Qiu ZJ. Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion. World J Gastroenterol 2009; 15(30): 3757-3766
URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3757.htm
DOI: https://dx.doi.org/10.3748/wjg.15.3757