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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jul 21, 2009; 15(27): 3355-3366
Published online Jul 21, 2009. doi: 10.3748/wjg.15.3355
Published online Jul 21, 2009. doi: 10.3748/wjg.15.3355
Figure 8 Effect of IGFBP-5 on signaling in serum-starved PaC cells.
BxPC-3 (A) and PANC-1 (B) stable transfectants [empty vector- (-), low IGFBP-5-L, or high IGFBP-5-H] were deprived of serum for 24 h then incubated in the presence of vehicle (DMSO), LY294002, or U0126 (10 &mgr;mol/L) for an additional 48 h. Cell lysates were prepared for immunoblot analysis of Akt or ERK1/2 activation using phospho-specific antibodies. Membranes were subsequently stripped and reprobed for total Akt or ERK1/2. Actin level was monitored to normalize protein loading. Quantitation of band density represents the level of IGFBP-5-associated expression relative to vector-transfected controls (1.0) normalized to the expression level of total Akt or ERK1/2. Results are representative of three independent experiments.
- Citation: Johnson SK, Haun RS. Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth. World J Gastroenterol 2009; 15(27): 3355-3366
- URL: https://www.wjgnet.com/1007-9327/full/v15/i27/3355.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.3355