Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth

Figure 7 IGFBP-5-conditioned medium enhances activation of Akt and ERK1/2 in PaC cells. IGFBP-5- (+) or vector-transfected (-) control cells were grown for 48 h in SFM. Conditioned medium was collected and applied to BxPC-3 or PANC-1 cells for indicated times. Cell lysates were prepared and immunoblot analysis of phosphorylated Akt or ERK1/2 was performed. Membranes were subsequently stripped and reprobed for total Akt or ERK1/2. Actin level was monitored to normalize protein loading. Quantitation of band density represents the level of IGFBP-5-associated expression relative to vector-transfected controls (1.0) normalized to the expression level of total Akt or ERK1/2. Results are representative of three independent experiments.