Pluronic L-81 ameliorates diabetic symptoms in db/db mice through transcriptional regulation of microsomal triglyceride transfer protein

Figure 3 L-81 regulated MTP transcription. A: The human MTP promoter was linked with luciferase reporter. Deletion or mutation constructs of MTP promoter-luciferase were transfected into HepG2 cells. The cells were treated with (black bars) or without (white bars) 1% L-81 in the medium. The luciferase activity in the cell lysate was measured. xxx = disabling mutations; B: L-81 inhibited apoB secretion in HepG2 cells. HepG2 cells were treated with the indicated concentration of L-81. The concentration of apoA-I and apoB in the culture medium were measured and normalized against the untreated control.