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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jun 28, 2009; 15(24): 3046-3050
Published online Jun 28, 2009. doi: 10.3748/wjg.15.3046
Published online Jun 28, 2009. doi: 10.3748/wjg.15.3046
Table 1 Sequences of primer pairs of Ca2+ channel subunits and RT-PCR
| Primer | Sequence | Temperature (°C) |
| L-α1C (5') | 5'-GTCCATAGTGGGTCGTCT-3' | 50 |
| L-α1C (3') | 5'-TGGTTTGTTCTTGCTTTC-3' | |
| L-α1D (5') | 5'-CAGGGATGCTGTGGAAGT-3' | 51 |
| L-α1D (3') | 5'-TGGGCTGAGAACCTAGACG-3' | |
| L-α-S (5') | 5'-ATGCCAGAGGATGACAACAAC-3' | 55 |
| L-α-S (3') | 5'-CACCCAgAAAgACAATGATgAA-3' | |
| L-α1F (5') | 5'-GACGGCAACTTGGCTTCT-3' | 53 |
| L-α1F (3') | 5'-GCTGGCATGACTGCTGGT-3' | |
| N-α1β(5') | 5'-GCTCGCTCTTCGTCTTCA-3' | 52 |
| N-α1β (3') | 5'-AGGTTCCGTGTCATCCAGT-3' | |
| R-α1E (5') | 5'-ATGTCCCTGAAGATGTATGG-3' | 50 |
| R-α1E (3') | 5'-AACGACCTCAAAGATGCTG-3' | |
| Ryr1 (5') | 5'-GGGCGGAGAATGAGAAAGA-3' | 55 |
| Ryr1 (3') | 5'-CAGGGTCGTCACGGTTGT-3' | |
| Ryr2 (5') | 5'-TGAGTATGCCCATTGAGT-3' | 48 |
| Ryr2 (3') | 5'-CTTTGCTTTAGGCGTGAG-3' | |
| TRPC1 (5') | 5'-GTCAGACATTAAGAGGCTGTG-3' | 50 |
| TRPC1 (3') | 5'-AAGTTGCCAAGTAAAGGGA-3' | |
| TRPC4α (5') | 5'-AATGGTTCTGCCCTGGTG-3' | 50 |
| TRPC4α (3') | 5'-GAAGATTTGGTTTGCGTTT-3' | |
| TRPC4β (5') | 5'-GCAGCATTCCTGGTCTCA-3' | 51 |
| TRPC4β (3') | 5'-GGGCGTGTTTCTCCTTTG-3' | |
| TRPM2 (5') | 5'-GTCATCACCATCGGCATAGC-3' | 56 |
| TRPM2 (3') | 5'-TGTCCAGGCAGGTCAGGTT-3' | |
| IP3R1 (5') | 5'-CAACCGTTACTATGGAAACATC-3' | 54 |
| IP3R1 (3') | 5'-TCAGCCAGGCTCATCTCAC-3' | |
| IP3R2 (5') | 5'-CGATGCCAGGATACGATGT-3' | 54 |
| IP3R2 (3') | 5'-CACCCTTGAAGTACCGATT-3' | |
| IP3R3 (5') | 5'-AGGAGCTGGTGGACGTGAT-3' | 55 |
| IP3R3 (3') | 5'-TGCTTGTTGTGCCTGGAAA-3' |
- Citation: Li F, Zhang ZM. Comparative identification of Ca2+ channel expression in INS-1 and rat pancreatic β cells. World J Gastroenterol 2009; 15(24): 3046-3050
- URL: https://www.wjgnet.com/1007-9327/full/v15/i24/3046.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.3046