Alisol B acetate induces apoptosis of SGC7901 cells via mitochondrial and phosphatidylinositol 3-kinases/Akt signaling pathways

Figure 6 Effect of Alisol B acetate on the expression of Bcl-2, Bax, Apaf-1, cleaved caspase-3 and caspase-9. SGC7901 cells were treated with 30 &mgr;mol/L alisol B acetate for 12, 24, 48 and 72 h. The cells were collected and lysed. Western blotting analysis was conducted and probed with antibodies to Bcl-2, Bax, Apaf-1, caspase-3 and caspase-9. Lanes (from left to right): Control cells; 12 h; 24 h; 48 h; 72 h.