Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo

Figure 3 YSA targets adenovirus to cells expressing murine EphA2R. A: Western blotting demonstrates that mouse 3T3 and Hep1-6 cells, like human pancreatic cancer cell lines Capan-1 and MIA PaCa-2, express the EphA2R. EphA2R was detected using a monoclonal antibody and the housekeeping protein actin was used as a loading control. B: Efficient transduction of mouse 3T3 and Hep1-6 cells by YSA-retargeted adenoviral vector. Cells were transduced with 1000 gc/cell of wt-Ad-Luc (white bars), Ad-Luc/∆F(FG)∆P (gray bars) or Ad-Luc/∆F(FG)∆P-YSA (black bars). Luciferase was measured after 24 h. Data are expressed as mean ± SD (n = 3).