Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Figure 2 Flow cytometry analysis of ICAM-1 and E-cadherin expression in SW1116. A: ICAM-1 (Room air control); B: ICAM-1 (0 h after the 6 mmHg insufflation); C: ICAM-1 (72 h after the 6 mmHg insufflation); D: E-cadherin (Room air control); E: E-cadherin (24 h after the 9 mmHg insufflation); F: E-cadherin (72 h after the 9 mmHg insufflation). Immediately after the 6 mmHg CO₂ insufflation (B), ICAM-1 expression increased significantly compared to room air controls (A) (P = 0.009), while it was significantly less at 72 h after the 6 mmHg insufflation (C) than control group. Twenty four hours after 9 mmHg CO₂ insufflation (E), E-cadherin expression increased significantly compared to controls (D) (P = 0.035), but returned to the control value at 72 h after the 9 mmHg insufflation (F).