Relationship between oxidative stress and hepatic glutathione levels in ethanol-mediated apoptosis of polarized hepatic cells

Figure 2 The relationship of CYP2E1 expression, oxidative stress, and WIF-B cell death following ethanol administration. WIF-B cultures were treated without (C) or with pyrazole (Cpyr) for 4 d followed by ethanol treatment for 48 h [E and E (pyr)]. A: CYP2E1 activity in isolated microsomes analyzed using the r-nitophenol oxidation assay in four independent experiments; B: H₂O₂ production representative of ROS generation detected by dichlorofluorescein production, and the activation of caspase-3 as a measure of apoptosis from seven independent experiments; C: Necrotic cell death evaluated by flow cytometric analysis in three independent experiments using the LIVE/DEAD cell stain as described in “Material and Methods”. ^aP < 0.05 vs control; ^cP < 0.05 vs ethanol alone-treated cells.