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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jun 7, 2009; 15(21): 2595-2601
Published online Jun 7, 2009. doi: 10.3748/wjg.15.2595
Published online Jun 7, 2009. doi: 10.3748/wjg.15.2595
Table 1 Methylation-specific PCR, real-time PCR primer, probe sequence and annealing temperature
| Gene | Primer purpose | Primer sequence (5'--3') | Annealing temperature (°C) | Product size (bp) |
| DKK-3 | MSP-M | F: GGGGCGGGCGGCGGGGC | 58 | 120 |
| R: ACATCTCCGCTCTACGCCCG | ||||
| MSP-U | F: TTAGGGGTGGGTGGTGGGGT | 56 | 126 | |
| R: CTACATCTCCACTCTACACCCA | ||||
| Real time RT-PCR | F: GTAAGTTTCCCCTCTGGCTTG | 60 | 90 | |
| R: AAGCACCAGACTGTGAAGCCT | ||||
| Probe: FAM+AGGTGTTGTGCATTTGTTCAGCTCCC+TAMRA | ||||
| WIF-1 | MSP-M | F: CGTTTTATTGGGCGTATCGT | 57 | 145 |
| R: ACTAACGCGAACGAAATACGA | ||||
| MSP-U | F: GGGTGTTTTATTGGGTGTATTGT | 52 | 154 | |
| R: AAAAAAACTAACACAAACAAAATACAAAC | ||||
| Real time RT-PCR | F: TCCAAACACCTCAAAATGCTATC | 60 | 119 | |
| R: GAACCCATCAGGACACTCGC | ||||
| Probe: FAM+ACAAGCTGAGTGCCCAGGCGG+TAMRA | ||||
| GAPDH | Real time RT-PCR | F: CCACTCCTCCACCTTTGAC | 60 | 102 |
| R: ACCCTGTTGCTGTAGCCA | ||||
| Probe: FAM+TTGCCCTCAACGACCACTTTGTC+TAMRA |
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Citation: Ding Z, Qian YB, Zhu LX, Xiong QR. Promoter methylation and mRNA expression of
DKK-3 andWIF-1 in hepatocellular carcinoma. World J Gastroenterol 2009; 15(21): 2595-2601 - URL: https://www.wjgnet.com/1007-9327/full/v15/i21/2595.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.2595