Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81

Figure 3 Interaction between tupaia CD81 LEL and HCV E2 protein. Plates were coated with 100 &mgr;L of recombinant GST or GST-CD81 LEL fusion proteins (5 mg/L), reciprocally diluted anti-GST antibody (A) (starting dilution at 1:1000) or HCV E2 protein (B) (starting concentration 2 mg/L) was added to the plates. Binding of anti-GST or HCV E2 protein was assessed as described in Materials and Methods. OD: optical density; Tup: tupaia; h: human; AGM: African green monkey; hCD81T163A: human CD81 containing a mutation of T to A at amino acid 165.