Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81

Figure 2 Expression and purification of soluble tupaia CD81 LEL using mouse monoclonal anti-human CD81 5A6 (A), and 1D6 (B) antibodies. Tupaia CD81 LEL was expressed and purified in E. coli as a GST fusion protein as described in Materials and Methods. Purified proteins were subjected to SDS-PAGE, and immunoblot using mouse monoclonal anti-human CD81 5A6 and 1D6 antibodies. Tup: tupaia; h: human; AGM: African green monkey; hCD81 LEL T163A: human CD81 containing a mutation of T to A at amino acid residue 163; 1: GST; 2: TupCD81 LEL-GST; 3: hCD81 LELT163A-GST; 4: AGMCD8 LEL-GST; 5: hCD81 LEL-GST.