New techniques in the tissue diagnosis of gastrointestinal neuromuscular diseases

Figure 3 Immunohistochemistry using antibodies to. A: Neuron specific enolase allowing clear visualisation of myenteric ganglia, neuronal number and size; B: Smooth muscle alpha actin showing absent staining in the circular muscle layer of the jejunum (arrows) in a patient with enteric dysmotility; C: CD3 showing small numbers of periganglionic T lymphocytes (arrows) in numbers that most would deem abnormal and indicative of ganglionitis; D: CD117 staining showing normal myenteric plexus interstitial cells of Cajal (ICC-MP). (Original magnification × 40-100).