Role of bacterial and genetic factors in gastric cancer in Costa Rica

Table 1 PCR primers for amplification of cagA, vacA and babA2 genes

Region	Primer	Nucleotide sequence	Reference
cagA	D008	5’-ATAATGCTAAATTAGACAACTTGAGCGA-3’	[28]
	R008	5’-TTAGAATAATCAACAAACATCACGCCAT-3’
	cagAFnz3	5’-AAAAGCGACCTTGAAAATTCC-3’	[29]
	cagA-seqR1	5’-TAGCATAATTGTCCAATTTCGC-3’
vacA s1	VA1-F	5’-ATGGAAATACAACAAACACAC-3’	[30]
	VA1-R	5’-CTGCTTGAATGCGCCAAAC-3’
vacA s1a	S1A-F1	5’-TCTYGCTTTAGTAGGAGC-3’	[30]
vacA s1b	SS3-F1	5’-AGCGCCATACCGCAAGAG-3’	[30]
vacA s1c	S1C-F1	5’-CTYGCTTTAGTRGGGYTA-3’	[13]
vacA s2	VA1-F1	5’-ATGGAAATACAACAAACACAC-3’	[30]
vacA m1	VA3-F	5’-GGTCAAAATGCGGTCATGG-3’	[30]
	VA3-R	5’-CCATTGGTACCTGTAGAAAC-3’
vacA m2	VA4-F	5’-GGAGCCCCAGGAAACATTG-3’	[30]
	VA4-R	5’-CATAACTAGCGCCTTGCAC-3’
babA2	babA-F	5’-AATCCAAAAAGGAGAAAAAGTATGAAA-3’	[31]
	babA-R	5’-TGTTAG TGATTTCGGTGTAGGACA-3’
	babA2-Fnc1	5’-GAAAAAACATGAAAAAACACATCCTTTCAT-3’
This study
	babA2-Rmn2	5’-TCTGGGTTAATGGCTTGCC-3’

¹Used with primer VA1-F.