Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates

Table 1 Primer used for PCR genotyping of Cuban H pylori strains

Primer	Sequence (5’-3’)	AT °C	Size (bp)	Ref.
glmMF	CCCTCACGCCATCAGTCCCAAAAA	60	417	[18]
glmMR	AAGAAGTCAAAAACGCCCCAAAAC
cagF1	GATAACAGGCAAGCTTTTGA	60	349	[7]
cagB1	CTGCAAAAGATTGTTTGGCAGA
vacAsF	ATGGAAATACAACAAACACAC	52	s1-259/s2-286	[20]
vacAsR	CTGCTTGAATGCGCCAAAC
vacAmF	CAATCTGTCCAATCAAGCGAG	56	m1-567/m2-642	[20]
vacAmR	GCGTCAAAATAATTCCAAGG
bab7-F	CCAAACGAAACAAAAAGCGT	60	271	[21]
bab7-R	GCTTGTGTAAAAGCCGTCGT
babA2F1	AATCCAAAAAGGAGAAAAAGTATGAAA	60	832	[13]
babA2R	TGTTAGTGATTTCGGTGTAGGACA
babA2R6072	GTTTTCTTTGAGCGCGGGTAAGC	60	607	[14]

¹Forward primer used with primer babA2R or babAR607 to amplify babA2 gene;

²Five nucleotides (GTTTT) were added to the original primer designed by Zambon et al[14] to increase specificity.