Association between Bmi1 and clinicopathological status of esophageal squamous cell carcinoma

Figure 1 Amplification curve (A) and standard curve (B) of quantitative real-time PCR for Bmi1 mRNA. Total RNA was extracted for subsequent reverse transcription, and standard cDNA was serially diluted to obtain five standard solutions (1 × 10^-1, 1 × 10^-2, 1 × 10^-3, 1 × 10^-4, 1 × 10^-5) for use in PCR to generate the reference curve, slope rate of the straight line (a) = -3.42089, intercept (b) = 5.97517, correlation coefficient (r) = 0.9996. The strength of Bmi1 and β-actin was directly generated by the machine.