Signal transduction mechanism of TRB3 in rats with non-alcoholic fatty liver disease

Figure 2 Fluorescent quantitative PCR for measurement of TRB3 mRNA expression levels. Real-time PCR was used for absolute quantification analysis. A: Amplification curve with the threshold value set in the exponential growth phase; B: Melting curve showing no primer dimer in PCR reaction; C: Standard curve for the relation (r = 0.99) between amplified results of PCR and Ct value of standard samples was in accordance with the requirements of real-time PCR. The intensities of fluorescent signals indicated the variation of product concentrations.