Involvement of 90-kuD ribosomal S6 kinase in collagen type I expression in rat hepatic fibrosis

Figure 6 Effect of p90RSK on collagen type I promoter activity. HSCs transfected with the pAVU6-siRSK (bar 3, to decrease p90RSK expression), pMT2·RSK1 (bar 5, to increase p90RSK expression) showed no alteration of collagen type I promoter activity compared to control cells sham-transfected with pAVU6+27 (bar 4), pMT2 (bar 6), respectively. HSCs transfected with collagen type I luciferase reporter construct (bar 1) as normal control and Renilla luciferase expression construct (bar 2) as positive control. After 24 h incubation, the luciferase activity was determined. Data represent luciferase activity relative to the control (bar 1) and are expressed as mean ± SD of three separate experiments.