Bile-acid-activated farnesoid X receptor regulates hydrogen sulfide production and hepatic microcirculation

Figure 1 CSE gene expression is regulated by bile acids. A, B: Qualitative and quantitative PCR showing the up-regulation of CSE mRNA in HepG2 cell line stimulated with FXR ligand 6E-CDCA (10 &mgr;mol/L) for 18 h. Data are shown as mean ± SD of three experiments. ^aP < 0.05 versus not stimulated cells; C: Western blotting analysis showing the up-regulation of CSE protein in HepG2 cell line stimulated by 6E-CDCA 10 &mgr;mol/L for 18 h; D, E: Immunohistochemistry analysis of CSE expression in HepG2 cells non-treated (D) and treated (E) with 6E-CDCA (10 &mgr;mol/L) for 18 h (Magnification × 40); F: Negative control was obtained by cell staining only with the secondary antibody.