Overexpression of DNA methyltransferase 1 and its biological significance in primary hepatocellular carcinoma

Figure 3 Inhibition of DNMT1 affects cell growth in HCC cell lines. Expression of endogenous DNMT1 protein in 7721-MT1 cells transfected with DNMT1 RNAi construct were decreased more than 80%. A: The SMMC-7721 cells were transfected transiently with DNMT1 siRNA and analyzed for their survival by trypan blue staining assay. The assays were performed in triplicates and the data are the mean ± SE; B: Western blotting analysis of DNMT1 expression in cells transfected transiently with DNMT1 siRNA for 1, 3, 5, 7 d; C: The SMMC-7721 cells were transfected stably with DNMT1 siRNA for 2 mo and the inhibition of DNMT1 and expression of PCNA were evaluated.