Expression and location of α-fetoprotein during rat colon development

Figure 2 Expression of AFP protein using Western blot analysis in the developing rat colons as indicated in lanes e18. 5, e20.5, P0, P7, P14, P21 and adult rats. A: Western blot analysis using AFP (C-19), an affinity purified goat polyclonal antibody against a peptide mapping at the C-terminus of AFP, revealed a 72-kDa isoform. The highest levels of AFP protein were detected in colons of rats at e18.5 and declined steadily during rat development and were undetected in adult rats. B: Results are indicated in percentage above the e18.5 value and are representative of three independent experiments. ^aP < 0.05 vs e18.5, e20.5 and P0, respectively.