Biotransformation of aesculin by human gut bacteria and identification of its metabolites in rat urine

Figure 1 Biotransformation of aseculin by anaerobic human gut bacteria. The gut bacteria comprised 20 pooled fecal samples from healthy volunteers. Incubation was performed under anaerobic conditions (80% N2, 10% CO₂, 10% H2) at 37°C for defined time periods. Ten millilitre of samples was collected at 0, 2, 4, 8, 12, 16, 24, 48 and 72 h, respectively post-incubation. Aesculin and metabolites were then extracted with methanol and analyzed by HPLC. The vertical axis shows the relative values for the concentration of aesculin and/or its metabolite. The horizontal axis indicates the HPLC overflow time.