Transforming growth factor-β1 induces intestinal myofibroblast differentiation and modulates their migration

Figure 6 Quantitative mRNA analyses of FN (A) and the splicing forms FN ED-A (B), and FN ED-B (C) by real-time PCR in CLPF treated with TGF-β1. Control CLPF (n = 7) were pre-incubated with and without 10 ng/mL TGF-β1 for 6 d, the monolayer wounded with a comb, and incubated for further 4 h with increasing concentrations of PDGF-AB in conditioned medium. mRNA was isolated and cDNA transcribed. cDNA start concentration of the untreated control (0 ng/mL TGF-β1 and 0 ng/mL PDGF-AB) was set as 1. Paired t-test: ^aP < 0.005; ^bP < 0.01; ^cP < 0.05.