Transforming growth factor-β1 induces intestinal myofibroblast differentiation and modulates their migration

Figure 4 Quantitative mRNA analysis of FN (A) and the splicing forms FN ED-A (B) and FN ED-B (C) by real-time PCR in TGF stimulated CLPF. TGF-β1 stimulated and untreated control-CLPF (n = 7) were lysed and mRNA was isolated. The cDNA start concentration of the untreated control group was set as 1. Compared to the control cells a dose-dependent increase in mRNA expression of FN, FN ED-A, and FN ED-B was determined. TGF-β1-stimulation for 2 d caused a higher increase in FN mRNA expression than after 6 d. Paired t-test: ^aP < 0.005; ^bP < 0.01; ^cP < 0.05.