Transforming growth factor-β1 induces intestinal myofibroblast differentiation and modulates their migration

Figure 3 Effect of TGF-β1 on growth factor-induced migration of CLPF. Control-CLPF were treated for 6 d with 10 ng/mL TGF-β1 and subsequently migration assays in the presence of PDGF-AB, IGF-I, EGF and TGF-β1 diluted in 24 h CLPF-conditioned medium were performed in the modified Boyden chamber. TGFβ1 treatment of CLPF significantly decreased the migratory potential, which could not be re-enhanced by cell migration-inducing growth factors. Statistics: The Mann-Whitney Rank Sum Test was used for the evaluation of the non-parametric data. A: ^bP < 0.0001 vs unstimulated CLPF; B: ^cP < 0.05, ^dP < 0.005 vs unstimulated CLPF; C: ^eP < 0.05 vs unstimulated CLPF; D: ^gP < 0.05, ^hP < 0.01 vs unstimulated CLPF.