Progression of diethylnitrosamine-induced hepatic carcinogenesis in carnitine-depleted rats

Figure 2 Photomicrographs of liver specimens stained with H&E. A: Liver from control rat showing normal liver histology with unremarkable central vein (A1, × 20 and A2, × 40); B: Liver from rat treated with DENA showing central vein surrounded by extensive necrosis and inflammatory infiltrate (B1, × 20), considerable hepatocyte necrosis represented with arrows (B2 and B3, × 40) and portal tract with bile duct proliferation and marked atypia (B4, × 20); C: Liver from rat treated with DENA plus D-carnitine-mildronate showing diffuse bridging fibrosis and nodule formation (C1, × 10) and bile ducts with marked reactive atypia showing nuclear enlargement, high nuclear/cytoplasmic ratio and prominent nucleoli (C2 and C3, × 40); D: Liver from rat treated with DENA and L-carnitine showing normal liver (D1, × 20) with unremarkable central vein (D2, × 40) and hepatic lobule with a focus of inflammatory infiltrate but no necrosis (D3, × 40).