Increased hepcidin expression in colorectal carcinogenesis

Figure 4 Cellular localization of hepcidin in colorectal cancer tissue. A: To determine hepcidin cellular localization in colorectal tissue immunohistochemistry was performed with a hepcidin specific antibody (Abcam 31877). (Aa) Human Liver; (Ab) Human Liver, incubated with both hepcidin antibody and immunizing hepcidin peptide; (Ac) Normal colon; (Ad) Normal colon with crypts in cross section; (Ae) Colorectal cancer (x 20); (Af) Colorectal cancer (x 40); (Ag) colorectal cancer (x 20); (Ah) Colorectal cancer (x 40). Boxes denote area subsequently magnified; B: Immunocapture of urinary hepcidin. (Ba) A human urine sample containing hepcidin 20 (m/z 2193.6), 22 (m/z 2438.2) and 25 (m/z 2792.0) was subject to immunocapture with either (Bb) protein G sepharose or with (Bc) protein G-sepharose and a polyclonal anti hepcidin antibody. Immunocaptured proteins were then eluted from the protein G sepharose and analysed by MALDI-TOF MS.