Clinical significance of loss of heterozygosity for M6P/IGF2R in patients with primary hepatocellular carcinoma

Table 1 M6P/IGF2R LOH analysis of paraffin-embedded tissue

Position	Nucleotide	Genotype	Amplicon size (bp)	F1 primer (5’-3’)	R1 primer (5’-3’)	Nested primer (5’-3’)
Exon 6	901	C/G	91	CACCAGGCGTTTGATGTTGG	CTCCAGCAAGGACCTGACTTTC	CCTCCGATGCTGTTGGCGT
Exon 9	1197	A/G	123	ACTAAGTAAGACTGTAATCTTCTAATACC	GTCTGTGGAGAAACTG AAATACAG	AATACCTATTCATATAAAACAAGCCTC
Exon 12	1737	G/A	111	TATTTGTCACAGAGTGCTGCAGG	GGCATCCAGTTTGGAATGAGTTAG	GGAAGATCTAGGTGATGCTTTTC
Exon 16	2286	A/G	187	GAAGCTTTCATATTATGATGGGATG	GAGGATACTCATGCCTGTGGTG	CATCGCGCTCCCTGAGGATACT
Exon 40	6206	A/G	118	GGGTGTGATGTGACATTTGAGTGG	GCCTTCCCAGTCCACCCGC	GGAGTGCAAATTCGTCCAGAAAC
Exon 47	X47-5	t/a	161	ATGCCCTCTCTACACTGGAGTA	GTAAGCTGACCACTTGCTGTAGG	CAGTGATAAGTAAGCTGACC