Infant intestinal Enterococcus faecalis down-regulates inflammatory responses in human intestinal cell lines

Table 2 PCR Primers and the annealing temperatures used for PCRs

Gene name	Forward	Reverse	Annealing temp (°C)	Length (bp)
β-actin	GGCGACGAGGCCCAGAGCAAGAGAGGCAT	CGATTTCCCGCTCGGCCGTGGTGGTGAAGC	55	460
IL-4	AACACAACTGAGAAGGAAACCTTC	GCTCGAACACTTTGAATATTTCTC	55	276
IL-8	ATGACTTCCAAGCTGGCCGTGGCT	TCTCAGCCCTCTTCAAAAACTTCTC	55	289
IL-10	ATGCCCCAAGCTGAGAACCAAGACCCA	TCTCAAGGGGCTGGGTCAGCTATCCCA	55	352
TGF-β	TGACAGCAGGGATAACACACT	GTAGGGGCAGGGCCCGAGGCA	55	288
TLR1	CTATACACCAAGTTGTCAGC	GTCTCCAACTCAGTAAGGTG	55	219
TLR2	GCCAAAGTCTTGATTGATTGG	TTGAAGTTCTCCAGCTCCTG	55	346
TLR3	GATCTGTCTCATAATGGCTTG	GACAGATTCCGAATGCTTGTG	55	304
TLR4	CTTTATCCAACCAGGTGC	GGAATGCTGGAAATCCAG	50	650
TLR5	TAGCTCCTAATCTGATG	CCATGTGAAGTCTTTGCTGC	50	437
TLR9	GTCCCCACTTCTCCATG	GGCACAGTCATGATGTTGTTG	55	259
TNF-α	CAGAGGGAAGAGTTCCCCAG	CCTTGGTCTGGTAGGAGACG	55	324
Tollip	CGCGGTACCGCCACCATGGCGACCACCGTCAGC	GCCGGGCCCTGGCTCCTCCCCCATCTG	60	850
TRAF6	GTCGGTACCGCCACCATGAGTCTGCTAAACTGTGAA	CGCGGGCCCCTATACCCCTGCATCAGTAC	60	1600