Berberine reverses free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKβ

Figure 5 Effect of Ber on the whole NF-κB p65 and nucleic NF-κB p65 protein expression in 3T3-L1 Adipocytes. Fully differentiated cells were cultured for 12 h in serum-free DMEM with 0.2% BSA and then they were, respectively, cultured for 24 h in DMEM containing 0.5 mmol/L PA and 1% BSA (Mod); or cultured for 24 and 48 h in DMEM containing 0.5 mmol/L PA, 10 &mgr;mol/L Ber,1% BSA (BH24, BH48); or in DMEM containing 0.5 mmol/L PA, 1 &mgr;mol/L Ber, 1% BSA for 24 h and 48 h (BL24, BL48); or cultured in DMEM containing 0.5 mmol/L PA, 5 mmol/L aspirin, 1% BSA for 24 h and 48 h (As24, As48); or in DMEM containing 1% BSA for 24 h (Nor).whole NF-κB p65 and nucleic NF-κB p65 protein expression were determined in the whole cell lysate and nucleic cell lysate by immunoblotting respectively. Each experiment was repeated three times. ^aP < 0.05 vs Mod group.