Synthesis of platelet-activating factor and its receptor expression in Kupffer cells in rat carbon tetrachloride-induced cirrhosis

Figure 2 A: Saturation curve binding to cirrhotic and normal rat Kupffer cells. Cells were used after overnight culture for receptor binding assay as described in the Method section. ³H-PAF at a concentration between 0.125 and 3.2 nmol/L was incubated with Kupffer cells in presence or absence of 5 &mgr;mol/L unlabeled PAF at 22°C for 3 h. ^aP < 0.05, ^bP < 0.01 vs control; B: Scattered blot analysis of ³H-PAF binding to cirrhotic and normal rat Kupffer cells. The results were analyzed by scatchard plot. Cirrhosis: R = 0.99, Kd = 2.08 nmol/L, Bax = 27.1882 ± 2.0885 fmol/mg. DNA; Control: R = 0.96, Kd = 1.57 nmol/L, Bax = 4.4024 ± 0.3155 fmol/mg. DNA.