Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice

Table 3 Quantification of gene expression by real-time quantitative PCR assay

Gene name	UniGene cluster	Primer sequences (5’-3’)		Combining sites (bp)	Amplifiers (bp)	WCA (120 mg/mL) vs NS control
						ΔΔCT (n)	2-ΔΔCT
stat3	Hs.421342	forward	CCTGGAGCAGCTCCATCAG	254	58	2.65 (4)	0.16 (0.11-0.24)
		reverse	AAACTGCCGCAGCTCCATT	311
RIPX	Hs.7927	forward	GAGTGCCTTTAAGCTGCAGAGTT	6	69	2.50 (5)	0.18 (0.13-0.23)
		reverse	TCCAAGCGACTGTTTAGTTCACTT	74
ROD1	Hs.269988	forward	AACTCCTCTCTGTAAAGCATTTTGC	525	64	2.09 (4)	0.23 (0.12-0.23)
		reverse	TGCACTGGGTCTTCTTTCAGAA	588
bcl-2	Hs.12677	forward	TGTTGGCCGGATCACCAT	2557	60	3.27 (5)	0.10 (0.06-0.17)
		reverse	TCCCCAATGATCAGGTCCTTT	2616