Bone marrow-derived dendritic cells pulsed with tumor lysates induce anti-tumor immunity against gastric cancer ex vivo

Figure 5 Assays for IFNγ secretion ex vivo. Splenic CD3⁺ T cells were isolated from naïve BALB/c mice with MACS. T cells were primed with SGC-7901 tumor cell lysate-pulsed BM-derived DCs as described in Materials and Methods. The primed T cells (effector cells) were mixied and incubated with SGC-7901 or B16 target cells. Supernatant from each well was collected for measuring IFNγ production with the mouse IFNγ ELISA kit. Statistical analysis used the paired Student’s t test. The results are representative of three independent experiments. Data are given as mean ± SE. ^bP < 0.01, SGC-7901 versus B16 or Non.