Bone marrow-derived dendritic cells pulsed with tumor lysates induce anti-tumor immunity against gastric cancer ex vivo

Figure 4 Generation of tumor-specific cytotoxic T cells ex vivo. Splenic CD3⁺ T cells were isolated from naïve B6 mice with MACS. T cells were primed with SGC-7901 tumor cell lysate-pulsed BM-derived DCs as described in Materials and Methods. Unpulsed DCs and SGC-7901 tumor lyastes were used as controls. The primed T cells (effector cells) were titrated by serial dilution (1:1, 5:1, 10:1, 25:1, 50:1, 100:1), and mixied with SGC-7901 or B16 target cells, and their lytic activity against SGC-7901 or B16 was assayed by a Cytotoxicity Detection Kit. Statistical analysis used the paired Student’s t test. The results are representative of three independent experiments. Data are given as mean ± SE. ^aP < 0.05, SGC-7901 TP DCs primed T cell/SGC-7901 group versus SGC-7901 TP DCs primed T cell/B16 group or other control groups.