Extent of liver resection modulates the activation of transcription factors and the production of cytokines involved in liver regeneration

Figure 6 Results of TUNEL staining from paraffin embedded tissue sections. Paraffin embedded tissue slides from regenerating rat liver were dewaxed, washed and stained using the TUNEL method. The slides were covered in anti-fade medium with DAPI and analysed with a fluorescence microscope. False positive results were ruled out by comparison of the images with DAPI counterstaining (not shown). The TUNEL index is the number of TUNEL-positive cells divided by the total cell number. Cells were counted in ten fields of vision per section. Numbers shown are mean of four separate experiments in each group. TUNEL index for control animals (no resection) was approximately 0.12%.