Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro

Figure 4 RT-PCR and Western blot analysis of differentiated WB-F344 cells treated or untreated with Wnt3a. A: Wnt3a-treated WB-F344 cells expressing two phenotypic markers (CK-19 and AFP) and two hepatic nuclear factors (HNF4α and HNF-6) at mRNA level with untreated WB-F344 cells as controls; B: Wnt3a-treated cells expressing two phenotypic markers (CK-19 and AFP) at protein level.