Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro

Figure 2 The mRNA expression levels of the known Wnt target genes CyclinD1 was semi-quantified at day 1 during stimulation with Wnt3a (160 ng/mL) and normalized to the expression levels in untreated WB-F344 cells (set as 100%). The mRNA expression level of CyclinD1, one of the known Wnt target genes in WB-F344 cells after stimulation by Wnt3a (160 ng/mL) for 1 d was semi-quantified by RT-PCR and the results was normalized to the expression levels in untreated WB-F344 cells (set as 1). CyclinD1 was upregulated under stimulating conditions on day 1. For quantification, CyclinD1 mRNA was scanned by densitometric analysis and normalized to GAPDH. Data are presented as mean ± SD of triplicate experiments. ^aP < 0.05 in comparison with un-treated cells.