Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro

Table 1 Primer sequences used for RT-PCR

Gene	Primer sequences	Annealing temp (°C)	Cycles	Ampliconsize (bp)
CyclinD1	5’-ATTGAAGCCCTTCTGGAGTCAAGCC-3’	56	26	420
	5’-TCTATTTTTGTAGCACCCCCCCGTC-3’
AFP	5’-GCTGAACCCAGAGTACTG AC-3’	60	34	472
	5’-GACACGTCGTAGATGAACGTG-3’
ALB	5’-AAGGCACCCCGATTACTCCG-3’	56	36	608
	5’-TGCGAAGTCACCCATCACCG-3’
CK-19	5’-ATGACTTCCTATAGCTATCG-3’	64	34	340
	5’-CACCTCCAGCTCGCCATTAG-3’
HNF-6	5’-GACAAATGGCAGGACGAGGG-3’	62	36	781
	5’-AGCGTACTGGTTTAGGTGCC-3’
HNF-4α	5’-CTTCCTTCTTCATGCCAG-3’	62	34	269
	5’-ACACGTCCCCATCTGAAG-3’
GAPDH	5’-ACCACAGTCCATGCCATCAC-3’	56	24	469
	5’-TCCACCACCCTGTTGCTGTA-3’