Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity

Figure 2 Cell-based luciferase assay for biological activity of ricin in CHO AA8 cells. A: Kinetics of induction of luciferase activity in CHO AA8 cells and inhibition by ricin and anisomycin. CHO AA8 cells were cultured in medium containing doxycycline (Dox) to suppress luciferase expression. For induction of luciferase expression, medium containing Dox was removed and replaced with medium (untreated) or medium containing ricin (1 μg/mL) or anisomycin (10 μg/mL). At various timepoints (0, 3, 6, 12, 24 h) after treatment, luciferase activity was measured. Light output is expressed as RLU; B: Dose-dependent inhibition of luciferase activity by increasing concentrations of ricin or anisomycin as control.