c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1

Figure 3 Nuclear Cyclin D1 stability is increased by c-Fos overexpression. IHH-C or IHH-Fos serum starved for 24 h were serum stimulated for 6 h and then treated with CHX (30 μg/mL) for 30 min, 1 h or 2 h. Nuclear extracts were immunoblotted with an antibody against Cyclin D1, and normalized with a SnRNP antibody, as indicated (Upper panels). The Cyclin D1 over SnRNP ratios were quantified by densitometric analysis of the immunoreactive bands (Lower panel). The results are the mean ± SE of three independent experiments.