c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1

Figure 2 Induction of cell cycle regulatory proteins after serum refeeding. IHH-C or IHH-Fos were serum starved for 24 h. Nuclear (A) or total (B) extracts prepared before or after serum stimulation for 8 h or 24 h were immunoblotted with antibodies, as indicated. Loading of nuclear or total extracts was normalized using a SnRNP or a β-actin antibody, respectively. Results of a representative experiment are shown (out of 3); C: Quantitative real time PCR of Cyclin D1 mRNA levels in quiescent IHH-C or IHH-Fos serum stimulated for the indicated times. Bars indicate mean ± SE of three independent experiments each performed in triplicate.