Ataxia telangiectasia-mutated-Rad3-related DNA damage checkpoint signaling pathway triggered by hepatitis B virus infection

Figure 2 HBV infection caused transient cell cycle arrest in the S and the G2 phase. The duration of the S and G2 phase were measured in HBV-infected or non-infected cells. Cells were synchronized with mimosine for 24 h, and then media were replaced with RPMI-1640 containing 10% HBV serum or normal serum. At indicated times, cells were harvested and examined for cell cycle profile using propidium iodide staining and flow cytometry. A: Cell cycle profile in HBV-infected cells and in non-infected cells; B: Percentages of cells in the S phase; C: Percentages of cells in the G2 phase. Mean and standard error are presented for three independent experiments.