Gli-1 siRNA induced apoptosis in Huh7 cells

Figure 5 Effect of Gli-1 siRNA combining with 5-Fu on Huh7 cells. The effect of Gli-1 siRNA combining with 5-Fu on Huh7 cell viability was measured by MTT assay. Cells were transfected with Gli-1 siRNA. After 6 h, cells were exposed to 5-Fu (25 &mgr;mol/L) or not for the indicated time periods. The bars are grouped according to the treatment time as labeled below the horizontal axis. The treatment groups were as labeled on the color key. Data are expressed as the mean ± SD of 3 independent experiments (^aP < 0.05 ; ^bP < 0.01 vs control or negative control).